B Raf(N581S)  [100 µL] Catalog Number:  CR-M26109 Gene Symbol:  BRAF, BRAF1, RAFB1 Description:   Anti BRaf(N581S) Mouse Monoclonal Antibody Background:   BRaf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. Immunogen:   A synthetic peptide from the internal region of Braf (N581S), human origin. Applications:   ELISA, WB, IHC, IP Recommended Dilutions:    ELISA: 1:1000-1:5000  WB: 1:500-1:2000  IHC: 1:100-200 Concentration:   0.5 mg/ml Host:   Mouse Format:   Liquid Clonality:   Monoclonal Isotype:    Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Recognizes B-Raf N581S, but not wild-type BRaf proteins from vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles. Western blot:

Western blot analysis of recombinant Braf proteins. Purified His-tagged BRaf(N581S) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti BRaf(N581S) monoclonal antibody (Cat. # CR-M26109).